Journal: bioRxiv

Article Title: Development of long-term oyster tissue cultures reveals cellular plasticity, regenerative potential, and sustained physiological activity

doi: 10.1101/2025.11.07.687009

Figure Lengend Snippet: (A-B) Hyalinocyte-like haemocytes from fresh haemolymph, characterized by dark cytoplasm, fibroblast-like adherence, and fine filopodia; cells often overlapped upon contact. (C) Granulocyte-like haemocytes from fresh haemolymph, displaying rounded morphology, distinct nuclei, and variable cytoplasmic granulation; cells typically aggregated margin-to-margin. (D-F) Granulocyte-like haemocytes shed from explant cultures: initial adhesion (D), limited granulation (E), or extensive granulation (F). (G-K) Hemacolor-stained haemolymph preparations showing cytoplasmic heterogeneity: hyalinocyte-like haemocytes without granulation (G, single plus), degranulated granulocytes without granulation (G, double plus; inset: thallium LUT highlighting cell margins), blast-like cells without granulation (H-I, asterisk) refractive eosinophilic inclusions (H, open arrowhead), fine basophilic granules (H, closed arrowhead), moderate basophilic granules (I, closed arrow), and coarse basophilic granules (J-K, open arrows). (L-N) Hemacolor-stained haemolymph preparations revealed heterogeneity in polymorphonuclear cells, including bilobed (L-M), trilobed (N) and multilobed (L) forms. Scale bars: (A-G) = 50 µm; (H-N) = 20 µm.

Article Snippet: For cytological staining, cells shed into the media (excluding non-adherent adipocytes from visceral mass explants) and fresh haemolymph were processed with the Hemacolor rapid staining system (Merck).

Techniques: Staining

Journal: bioRxiv

Article Title: Development of long-term oyster tissue cultures reveals cellular plasticity, regenerative potential, and sustained physiological activity

doi: 10.1101/2025.11.07.687009

Figure Lengend Snippet: (A-B) Ciliated cells shed into culture medium. (C) Mantle lobes in vivo . (D-E) Mantle lobe regeneration in culture; regrowing lobes (bracket), outer dead layer shed into medium (open arrow), explant (plus). (F) Mucus in culture (bracket), explant; gill (plus). (G) Mantle tissue stained with Masson’s trichrome modified with Alcian blue; mucocytes (closed arrow). (H) Gill tissue stained with Masson’s trichrome modified with Alcian blue; mucocytes (closed arrow) and epithelial-associated eosinophilic cell (asterisk). (I) Hemacolor staining of a mantle and gill-derived cell shed into culture medium; likely a mucocyte. (J) Hemacolor staining of an epithelial-associated eosinophilic cell shed from gill explant culture medium. (K) Unidentified round cell phenotypes. Granulocytes (open arrowhead) and myocytes (closed arrowhead). (L) Smooth muscle cells (myocytes) in early culture, showing elliptical structures formed by proliferating myocytes (M) Elongated myocytes shed into medium, arising from rounded cells shown in (L). (N) Myocytes in mantle tissue from cultured explant, H&E stain. (O-P) Shed myocytes from culture, Hemacolor stain. Scale bars: (A, H, O, P) = 20 µm; (B, G, K, L, M, N, Q) = 50 µm; (C-E) = 500 µm; (F) = 100 µm; (I, J) = 10 µm.

Article Snippet: For cytological staining, cells shed into the media (excluding non-adherent adipocytes from visceral mass explants) and fresh haemolymph were processed with the Hemacolor rapid staining system (Merck).

Techniques: In Vivo, Staining, Modification, Derivative Assay, Cell Culture